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MetaQUAST evaluates and compares metagenome assemblies based on alignments to close references. It is based on QUAST genome quality assessment tool, but addresses features specific for metagenome datasets:
- Huge species diversity – the tool accepts multiple references and makes multi-genome tables and plots, including Krona charts.
- Commonly unknown species content – the tool automatically detects and downloads reference sequences from NCBI.
- Presence of highly relative genomes – the tool detects chimeric contigs and reports "interspecies misassemblies" in addition to the regular assembly errors types.
MetaQUAST can be fed with multiple assemblies, thus is perfect for comparison.
MetaQUAST is distributed within QUAST package since version 2.2.
GitHub Full manual (MetaQUAST specific sections: running, output description)
Paper at Bioinformatics journal
Citation:
Alla Mikheenko, Vladislav Saveliev, Alexey Gurevich,
MetaQUAST: evaluation of metagenome assemblies,
Bioinformatics (2016) 32 (7): 1088-1090.
First published online: November 26, 2015
Examples of comparison of 4 assemblers on 3 datasets generated with MetaQUAST v3.2:
Please help us to make MetaQUAST better by sending your comments, bug reports, and suggestions to quast.support@bioinf.spbau.ru